TOXICOLOGICAL SCREENING: DETECTION OF AFLATOXIN B1 IN PET FOOD SAMPLES.

  • Talyanny Fernandes Franco UFRN
  • Herbert Ary Sisenando Universidade Federal do Rio Grande do Norte
  • Thaynara Mirelly Gomes Dantas Universidade Federal do Rio Grande do Norte
  • George Queiroz de Brito Universidade Federal do Rio Grande do Norte
  • Juliana Vilar Furtado de Medeiros Universidade Federal do Rio Grande do Norte
  • Zama Messala Luna da Silveira Universidade Federal do Rio Grande do Norte
Keywords: pet food;, aflatoxin;, thin-layer chromatography;, toxicology.

Abstract

Aflatoxin is a class of mycotoxin produced mainly by fungi of the genus Aspergillus sp. It is characterized by its high carcinogenic potential and is found mainly in foods used as a basis for animal nutrition products and even in the final product itself. This study aimed to identify the aflatoxin B1 presence in Pet food using the qualitative method aiming to associate the results obtained with extrinsic factors, such as humidity and temperature. Six specimens of pet food were used, classified according to its price, the type of animal for which it is intended, and the period they were acquired in the local market. Altogether, 12 samples were analyzed in duplicate. The extraction and purification method of this research was adapted from the methodology determined by the International Official Methods of Analysis (AOAC), which was efficient in tests that preceded the analysis of the study samples. For the detection of the toxin, the thin-layer chromatography was used, with subsequent chemical development using a 25% aqueous solution of sulfuric acid on the chromatographic plate, for analysis under ultraviolet light. Although the samples evaluated showed some marks in the chromatographic separation when using the chemical developer, it can be inferred that these did not express positive results regarding the aflatoxin B1 contamination. However, it is not possible to state that the samples are not contaminated since there is a need to use more sensitive analytical methods to identify this type of mycotoxin.
Published
2022-02-02
How to Cite
Fernandes Franco, T., Ary Sisenando, H., Gomes Dantas, T. M., Queiroz de Brito, G., Vilar Furtado de Medeiros, J., & Messala Luna da Silveira, Z. (2022). TOXICOLOGICAL SCREENING: DETECTION OF AFLATOXIN B1 IN PET FOOD SAMPLES, 15(1), 17-27. https://doi.org/10.22280/revintervol15ed1.505