Proposal of open field test as a model of varenicline pharmacokinetic study in rats

  • Julia Zaccarelli-Magalhães Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo
  • Thaisa Meira Sandini Faculdade de Ciências Farmacêuticas da Universidade de São Paulo
  • André Rinaldi Fukushima Universidade São Judas Tadeu
  • Helenice de Souza Spinosa Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo
Palavras-chave: Nicotinic Cholinergic Receptors, Open Field, Pharmacokinetic, Varenicline.

Resumo

Varenicline is a medication used for smoking treatment that acts as a partial agonist for nicotinic cholinergic receptors α4β2 and α3β4 and as a total agonist of receptor α7 in the central nervous system. Pharmacokinetic is important information for medications that acts in the central nervous system. This kind of assay is commonly done by expensive and complex analytical techniques. Therefore, the aim of this study was to evaluate the possibility of using the open field test as a pharmacokinetic model for varenicline in male rats exposed to a single dose of varenicline. Male rats received a single dose orally (gavage) of three different concentrations of varenicline: 0.03 (therapeutic dose for humans), 0.1 and 0.3 mg/kg or water (control group). The open field observations were recorded 30 min, 1, 2, 4, 6, 24, 48, 72 h and 7 days after the administration of varenicline or water. The results showed alterations in locomotion and rearing frequencies, as well as in immobility time observed in open field, which is consistent with this drug’s plasma peak. Consequently, this behavioral test apparently can be considerate as a model for pharmacokinetic evaluation of varenicline.

Biografia do Autor

Julia Zaccarelli-Magalhães, Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo
Possui graduação em Bacharelado no curso de Ciências Biológicas da Universidade Presbiteriana Mackenzie (2014). Possui título de mestre em Ciências pela Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (2016). Atualmente é aluna de doutorado no Programa de Patologia Experimental e Comparada na Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo.

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Publicado
2018-10-31
Como Citar
Zaccarelli-Magalhães, J., Meira Sandini, T., Rinaldi Fukushima, A., & de Souza Spinosa, H. (2018). Proposal of open field test as a model of varenicline pharmacokinetic study in rats. Revista Intertox De Toxicologia, Risco Ambiental E Sociedade, 11(3). https://doi.org/10.22280/revintervol11ed3.398
Seção
FARMÁCIA (ANÁLISE TOXICOLÓGICA)